摘要 :
Currently used clinical and histopathological parameters imprecisely define the risk of distant recurrence in breast cancer, underscoring the need for more informative prognostic markers. In the present fluorescence in situ hybrid...
展开
Currently used clinical and histopathological parameters imprecisely define the risk of distant recurrence in breast cancer, underscoring the need for more informative prognostic markers. In the present fluorescence in situ hybridization study of archived surgical specimens, we derived an algorithm for computing a prognostic index (PI) from DNA copy numbers of three genomic regions (CYP24, PDCD6IP, and BIRC5) for estrogen/progesterone receptor-positive (ER/PR+) cancers and a distinct PI (based on NR1D1, SMARCE1, and BIRC5) for estrogen/progesterone receptor-negative (ER/PR–) cancers. Among independent test cases stratified by PI, recurrence rates were significantly higher among high-risk patients than low-risk patients for both ER/PR+ (odds ratio = 9.52, 95% confidence interval >2.12, P = 0.0024) and ER/PR– (odds ratio = 12.3, 95% confidence interval >1.45, P = 0.0188) cancers. Among the entire population, recurrences were significantly more prevalent for cases with PI above the medians for both ER/PR+ (Fisher’s exact, P = 1.19 x 10–5) and ER/PR– (P = 0.0025) patients and for the node-negative subsets (ER/PR+ node-negative, P = 0.042 and ER/PR– node-negative, P = 0.039). In conclusion, these markers perform well in comparison with other criteria for recurrence risk assessment and can be used with routinely formalin-fixed, paraffin-embedded surgical specimens.
收起
摘要 :
The aim of this study was to assess the effect of bifenthrin on common carp (Cyprinus carpio L.). The 96-h LC50 value of Talstar EC 10 (active substance 100 g l(-1) bifenthrin) was found to be 57.5 mu g l(-1). Examination of haema...
展开
The aim of this study was to assess the effect of bifenthrin on common carp (Cyprinus carpio L.). The 96-h LC50 value of Talstar EC 10 (active substance 100 g l(-1) bifenthrin) was found to be 57.5 mu g l(-1). Examination of haematological and biochemical profiles and histological tissue examination was performed on common carp after 96 h of exposure to Talstar EC 10 (57.5 mu g l(-1)). The experimental group showed significantly higher (P < 0.01) values of plasma glucose, ammonia, aspartate aminotransferase and creatine kinase as well as the relative and absolute monocyte count, compared with the control group. Histological examination revealed teleangioectasiae of secondary gill lamellae and degeneration of hepatocytes. The bifenthrin-based Talstar EC 10 pesticide preparation was classified as a substance strongly toxic for fish.
收起
摘要 :
The aim of this study was to assess the effect of bifenthrin on common carp (Cyprinus carpio L.). The 96-h LC50 value of Talstar EC 10 (active substance 100 g l(-1) bifenthrin) was found to be 57.5 mu g l(-1). Examination of haema...
展开
The aim of this study was to assess the effect of bifenthrin on common carp (Cyprinus carpio L.). The 96-h LC50 value of Talstar EC 10 (active substance 100 g l(-1) bifenthrin) was found to be 57.5 mu g l(-1). Examination of haematological and biochemical profiles and histological tissue examination was performed on common carp after 96 h of exposure to Talstar EC 10 (57.5 mu g l(-1)). The experimental group showed significantly higher (P < 0.01) values of plasma glucose, ammonia, aspartate aminotransferase and creatine kinase as well as the relative and absolute monocyte count, compared with the control group. Histological examination revealed teleangioectasiae of secondary gill lamellae and degeneration of hepatocytes. The bifenthrin-based Talstar EC 10 pesticide preparation was classified as a substance strongly toxic for fish.
收起
摘要 :
The molecular characterization of endometrial carcinoma (EC) has recently been included in the ESGO/ESTRO/ESP guidelines. The study aims to evaluate the impact of integrated molecular and pathologic risk stratification in the clin...
展开
The molecular characterization of endometrial carcinoma (EC) has recently been included in the ESGO/ESTRO/ESP guidelines. The study aims to evaluate the impact of integrated molecular and pathologic risk stratification in the clinical practice and the relevance of pathologic parameters in predicting prognosis in each EC molecular subgroup. ECs were classified using immunohistochemistry and next-generation sequencing into the four molecular classes: POLE mutant (POLE), mismatch repair deficient (MMRd), p53 mutant (p53abn), and no specific molecular profile (NSMP). According to the WHO algorithm, 219 ECs were subdivided into the following molecular subgroups: 7.8% POLE, 31% MMRd, 21% p53abn, 40.2% NSMP. Molecular classes as well as ESGO/ESTRO/ESP 2020 risk groups were statistically correlated with disease-free survival. Considering the impact of histopathologic features in each molecular class, stage was found to be the strongest prognostic factor in MMRd ECs, whereas in the p53abn subgroup, only lymph node status was associated with recurrent disease. Interestingly, in the NSMP tumor, several histopathologic features were correlated with recurrence: histotype, grade, stage, tumor necrosis, and substantial lymphovascular space invasion. Considering early-stage NSMP ECs, substantial lymphovascular space invasion was the only independent prognostic factor. Our study supports the prognostic importance of EC molecular classification and demonstrated the essential role of histopathologic assessment in patients' management.
收起
摘要 :
Organophosphate pesticides, commonly used in large scale farming, have been found to be major contaminants in aquatic environment. Clarias gariepinus was exposed to acute and sublethal concentrations of phostoxin and DD Force to e...
展开
Organophosphate pesticides, commonly used in large scale farming, have been found to be major contaminants in aquatic environment. Clarias gariepinus was exposed to acute and sublethal concentrations of phostoxin and DD Force to evaluate single and joint action toxicity of the organophosphates. Effects of phostoxin and DD force on antioxidant enzymes, fish organs and acetylcholinesterase levels in fingerlings and juveniles of C. gariepinus were also investigated. The lethal concentrations (96 h LC50) for phostoxin and DD Force were 0.631 and 1.759 mg/l, respectively. The results obtained from the bioassay showed that phostoxin was 2.8x more toxic than DD Force after exposure of C. gariepinus. Joint action toxicity evaluations of phostoxin and DD Force showed that the interaction between the chemicals was synergistic (RTU>1). The biochemical responses in the exposed fish differed significantly (P<0.05) from the control fish. The result of acetylcholinesterase study revealed significant difference between acetylcholinesterase levels in the exposed fish and control, with reduction in the acetylcholineterase level in fish exposed to sublethal concentrations of phostoxin and DD Force. Haematological studies revealed an increase in WBC, RBC, PCV and platelets in the exposed fish. Histopathology of the gills showed shortened primary lamellae, loss of secondary lamellae and loss of ceratobrachial bones. In the acute toxicity studies, respiratory stress, erratic swimming and instant death of fish were observed in the exposed fish. This study reveals that changes in histopathology and acetylcholinesterase level are good biomarkers and can be successfully used to detect exposure to organophosphates pesticides in fish.
收起
摘要 :
To evaluate the hematological parameters in caprine besnoitiosis, blood samples from 24, each infected with severe besnoitiosis as well as normal goats, were compared. To identify the besnoitia positive goats, biopsies and tissue ...
展开
To evaluate the hematological parameters in caprine besnoitiosis, blood samples from 24, each infected with severe besnoitiosis as well as normal goats, were compared. To identify the besnoitia positive goats, biopsies and tissue samples were taken from mid region of the skin of the right tarsal and carpal joints of the goats that showed symptoms of besnoitiosis. Red blood cells, lymphocytes count, hemoglobin concentration and hematocrit values of the infected goats were significantly (P<0.05) lower and mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), white blood cells, neutrophils, eosinophils, monocytes and basophils counts were significantly (P<0.05) higher as compared to those of normal ones. Severity of infection effected differences in some of these parameters.
收起
摘要 :
Pulp Out, paste-contained jatropha sap, sidaguri roots, and melittin, has been studied to have potency used as an herbal-based devitalizing agent. Prior to clinical application, the toxicity of Pulp Out should be evaluated as it m...
展开
Pulp Out, paste-contained jatropha sap, sidaguri roots, and melittin, has been studied to have potency used as an herbal-based devitalizing agent. Prior to clinical application, the toxicity of Pulp Out should be evaluated as it might leak from the cavity unintentionally and get into the digestive systems, which can cause either local or systemic effects. The present study aimed to evaluate the impact of Pulp Out application on periodontal and periapical tissue as well as acute toxicity in Wistar rats. The paste was inserted into the periapical tissue. After 7 days, the periapical tissue was isolated for histopathological evaluation. Pulp Out in an oral suspension of 50, 500 and 2500?mg/kg BW was administered. Autonomic nerve signs were observed intensively every 4?h as well as water and food consumption for 14 days. Biochemical, hematologic parameters and specific organs were evaluated. Therefore, considering the inflammatory lymphocyte cells, osteoblasts, and osteoclasts, Pulp Out is not toxic. The acute toxicity study revealed no treatment-related death was observed, indicating that LD<sub>50</sub> is greater than 2500?mg/kg BW. No significant difference statistically either in body weight, water or food consumption as was observed in autonomic clinical signs of treated groups when compared to the control. Similarly, biochemical and hematologic properties showed no significant difference compared to control. Histopathological, slightly hydrophilic degenerative was observed in all organs. In conclusion, Pulp Out showed low acute toxicity in Wistar rats.
收起
摘要 :
Morinda lucida is used as a remedy for diabetes and some other diseases in African folk medicine. In an acute toxicity study, Morinda lucida extract was administered orally at doses ranging between 2-20 g kg~-1 to experimental mic...
展开
Morinda lucida is used as a remedy for diabetes and some other diseases in African folk medicine. In an acute toxicity study, Morinda lucida extract was administered orally at doses ranging between 2-20 g kg~-1 to experimental mice and observed for any toxic symptoms up to 14 days. In the sub-acute toxicity, Morinda lucida stem bark extract was tested at dose levels of 0.1, 1 and 5 mg kg~-1 on physical (body weight and organ weight), biochemical parameter and histopathological examination in adult male Albino rats. The Morinda lucida extract was well tolerated at the acute administration. No mortality was observed even at the highest dose of 20 g kg~-1. In the sub-acute administration, Morinda lucida extract significantly (p<0.05) prevented an increase in body weight in a dose dependent manner and this reflected a significant (p<0.05) increase in the relative organs (liver, heart, kidney and spleen) weights. The biochemical parameters showed a significant (p<0.05) increase in ALT and AST at the dose level of 5 g kg"1 in both two weeks and four weeks examination. Extract had a significant (5<0.05) dose reducing effect on creatinine and triglycerides at 2 weeks administration while at four weeks extract had significant reduction effect on glucose creatinine, cholesterol and triglyceride at dose level of 5 g kg~-1. The histopathological examination revealed steatosis in the liver at 5 g kg~-1Morinda lucida extract administration. Thus M. lucida aqueous extract is well tolerated at low dose administration but may be toxic at dose level of 5 g kg~-1 at sub-acute administration.
收起
摘要 :
Pesticide accumulates in aquatic ecosystems and exerts toxic effects on aquatic animals. In this study, stress parameters and tissue histopathology under acute diazinon exposure were investigated in fish, scat (Scatophagus argus)....
展开
Pesticide accumulates in aquatic ecosystems and exerts toxic effects on aquatic animals. In this study, stress parameters and tissue histopathology under acute diazinon exposure were investigated in fish, scat (Scatophagus argus). Spotted scat was exposed to different diazinon concentrations (0, 10, 20, 30 mu g L-1) for 24, 48, 72, 96 h. Cortisol and glucose levels showed a significant increase after exposure to different diazinon concentration with increase in exposure time. The electrolytes (K+, Cl-, Ca2+, Na+) and glucose were differentially affected during the exposure to diazinon. Gill and kidney tissues showed many histopathological changes in diazinon-exposed fish. These results suggest that the release of spotted scat (S. argus) into the diazinon-contaminated regions may alter their physiology and jeopardize their survival.
收起
摘要 :
Chlorpyrifos, an organophosphate insecticide, inhibits the enzyme acetylcholinesterse resulting in the accumulation of acetylcholine at the nerve endings. In the present research chlorpyrifos was administered via dermal contact to...
展开
Chlorpyrifos, an organophosphate insecticide, inhibits the enzyme acetylcholinesterse resulting in the accumulation of acetylcholine at the nerve endings. In the present research chlorpyrifos was administered via dermal contact to New Zealand white rabbits to determine its effects on biochemical indices on the rabbits as well as the pathological changes in their liver and brain. Twelve adult male rabbits were randomly assigned to two equal groups (N = 6; treatment and control group). The treatment group received chlorpyrifos via dermal contact at the doses of 50 mg per kilogram body weight everyday during the first week. The daily doses for the second, third, and fourth weeks were 100, 250, and 400 mg/kg body weight, respectively. The control group received ethyl alcohol as the solvent of chlorpyrifos during the same period. Blood samples from marginal ear vein of both groups were collected on days 0, 7, 14, 21, and 28. Statistical study revealed that chlorpyrifos has harmful effects on the biochemical indices of the treated rabbits. These changes included significant increase in the level of ALP, AST, ALT, and cholesterol in the serum (p < 0. 05, p < 0. 01). Also significant decrease was observed in triglyceride and total protein level of the serum in the treatment group as compared with the control group (p < 0. 05). On day 28, the rabbits in both groups were euthanized. Histopathological changes including necrosis of hepatocytes, infiltration of lymphocyte cells, hyperemia, and proliferation of fibroblasts were observed in liver tissue of the exposed rabbits. In addition, necrosis of neuron, encephalitis (perivascular cuffing), hyperemia in blood vessels, gliosis, and necrosis of Purkinje cells in cerebellum were seen in the brain sections. The study confirmed that organophosphate intoxication from chlorpyrifos can occur via dermal exposure. Dermal contact to chlorpyrifos can have toxic effects on biochemical indices. It can also have pathologic effects on the liver and brain tissue.
收起
原文获取